Determination of Enzyme Activities by Capillary Zone Electrophoresis

Authors

  • Z. Svagera Department of Analytical Chemistry,
  • T. Adam Centre of Analytical Chemistry of Molecular Structures, Medical Faculty, Palacky University, Olomouc
  • P. Bartak Centre of Analytical Chemistry of Molecular Structures, Medical Faculty, Palacky University, Olomouc
  • J. Sevcik Centre of Analytical Chemistry of Molecular Structures, Medical Faculty, Palacky University, Olomouc

Abstract

Capillary electrophoresis was applied for the determination of activities of purine-metabolism enzymes: purinenucleoside phosphorylase (PNP), adenine phosphoribosyl transferase (APRT), adenosine deaminase (ADA), and hypoxanthine guanine phosphoribosyl transferase (HPRT). The enzyme activities were determined in the lysate of human erythrocytes after incubation with the corresponding substrate. For clinical purposes, the developed methods were validated by comparison with the standard HPLC methods.

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Published

1998-09-15

How to Cite

Svagera, Z., Adam, T., Bartak, P., & Sevcik, J. (1998). Determination of Enzyme Activities by Capillary Zone Electrophoresis. Chemické Listy, 92(8). Retrieved from http://www-.chemicke-listy.cz/ojs3/index.php/chemicke-listy/article/view/2683

Issue

Vol. 92 No. 8 (1998)

Section

Articles